Elucidating biosynthetic pathways for vitamins and cofactors
It was observed that ATP, GTP, NAD, FAD and mannose preferred , , , and residues respectively, whereas vitamins preferred residues for the interaction with proteins.
It suggested that protein-binding patterns of vitamins are different from other ligands, and motivated us to develop separate predictor for vitamins and their sub-classes.
Furthermore, a substrate-carrier protein, Cob E, has been identified, which can also be used to stabilize some of the transient metabolic intermediates and enhance their onward transformation.
The tight association of pathway intermediates with enzymes provides evidence for a form of metabolite channeling.
The isolation and characterization of biochemical pathways for vitamins and coenzymes has often proved technically challenging, mainly due to the low abundance and kinetic lability of the metabolic intermediates.
The biosynthesis of the corrin-ring component is mediated via distinct aerobic and anaerobic pathways that oversee the transformation of the tetrapyrrole primogenitor, uroporphyrinogen III, into cobyric acid.).